A simple, precise and economic high-performance liquid chromatographic method was developed for estimation of\nclotrimazole in commercial semisolid formulations. Metronidazole was used as internal standard. The retention time of\nclotrimazole and metronidazole was 10.6 min and 3.2 min respectively. Calibration curve was linear in the range of 10-50\n�¼g/ml. The values of RSD determined for precision, accuracy and robustness studies were less than 2%. The limits of detection\nand quantification were found to be 1 �¼g/ml and 3 �¼g/ml respectively. The proposed validated method can be employed for\nroutine quality control analysis of vaginal formulations as per international conference on harmonization guidelines.
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